Yellow sugarcane aphid, Sipha flava (Forbes) (Hemiptera: Aphididae), widely distributed throughout the Americas and invasive in Africa, has been detected in Réunion Island since 2017. This insect causes damages to several crop species, including sugarcane (Saccharum spp.). On sugarcane, yellow to purple discoloration of leaves are frequently observed in commercial fields from the early growing stages. This study conducted in Réunion aimed at testing for the existence of genetic variability for resistance to S. flava in advanced sugarcane varieties. S. flava prevalence (SFP) was evaluated on an unbalanced set of 25 elite sugarcane breeding varieties that were tested in a multi-environment trial (MET) network of five locations. All trials consisted of randomised complete block designs, composed with four replicates. S. flava prevalence (SFP) was assessed on the lowest green leaf of 20 randomly chosen stalks, 2-3 months after trial planting. Individual trial analyses showed significant genetic variability for SFP. Genetic coefficient of variation (CVg) ranged between 13.5% and 22.6% depending on the trial. Intermediate (0.46) to high broad-sense (0.79) heritability values (H²) were found. A strong consistency of variety resistance was observed between pairwise environments (mean correlation of 0.64). At the MET level, the genetic coefficient of variation reached 15.0%, contribution of genotype x environment interaction was low (7.8%), and H² reached 0.73. These field evaluations confirmed the existence of a genetic variability in resistance to S. flava amongst the current elite breeding material of Réunion Island. They indicated that genetic improvement for S. flava resistance could be obtained through conventional breeding. Specific bioassays under controlled conditions would be needed to decipher precise mechanisms of resistance observed in this study.