This study is conducted to investigate the effect of contrast polarity towards eye fixation patterns when reading text on a smartphone in bright and dark conditions involving the effects when reading on a smartphone such as in real-life situations. The number of fixations and duration of fixation showed no statistically significant difference (p=0.160 and 0.099 respectively). However, emmetropic subjects showed a higher result in bright conditions compared to myopic subjects (p=0.046). This concludes that emmetropic eye movement efficiency seems superior, possibly due to lower spherical order aberration as pupil size decreases in bright illumination. Keywords: Contrast Polarity; Fixation Rates; Eye Tracking; Light conditions eISSN: 2398-4287 © 2023. The Authors. Published for AMER & cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), and cE-Bs (Centre for Environment-Behaviour Studies), College of Built Environment, Universiti Teknologi MARA, Malaysia DOI: https://doi.org/10.21834/ebpj.v8i24.4680