Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin
- Resource Type
- Authors
- Grazieli Maboni; Michael Agbaje; Sabine Tötemeyer; Melissa Bexon; Ceri E. Stewart; Michael Jones; Adam M. Blanchard; Catrin S. Rutland
- Source
- PeerJ
PeerJ, Vol 6, p e5097 (2018)
- Subject
- Veterinary Medicine
0301 basic medicine
medicine.medical_specialty
Pathology
Histology
Immunology
lcsh:Medicine
Histopathology
Virulence
Inflammation
Dichelobacter nodosus
Haematoxylin
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
Degenerative disease
medicine
Agricultural Science
Footrot
biology
business.industry
General Neuroscience
lcsh:R
General Medicine
Pro-inflammatory cytokine
medicine.disease
biology.organism_classification
Ovine
Footrot, Pro-inflammatory cytokine, Dichelobacter nodosus, Inflammation, Histopathology,Ovine
030104 developmental biology
chemistry
Lameness
medicine.symptom
General Agricultural and Biological Sciences
business
- Language
- ISSN
- 2167-8359
Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoof-horn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1β) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1β expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p D. nodosus load.