Microalgae and bacteria are part of the vital components of various ecosystems. Photosynthetic microalgae capture solar energy and convert it to bioenergy and biochemical products. They exude dissolved organic carbon that becomes available to bacteria. In return, the bacteria remineralize sulphur, nitrogen and phosphorous to support the further growth of microalgae. Microalgae-bacteria symbiosis represents commensal and mutualistic inter-kingdom relationships, that may result in stimulation of algal growth, but also in the development of new metabolites with a prominent potential for application in the industry sector. Chlorella vulgaris is a green unicellular microalga with numerous biological and pharmacological properties important for human health. Streptomycetes are the most thoroughly studied microorganisms producing biologically active compounds, such as antibiotics, anticancer agents, immunosuppressives, antifungals, etc. In the recent decade, co-cultivation, as a potential replacement for monoculturures of various microorganisms, gained special interest due to a whole range of desirable characteristics, such as modularity, robustness, predictability, scalability and stability. However, very little is known about the significance of S. rimosus and C. vulgaris co-cultures concerning the production of high-value metabolites with anti-bacterial, anti- oxidant, and anti-aging activities. To determine the significance of their co-cultivation, a rangeseries of biological assays were performed on algal and bacterial monocultures, as well as co- culture. The antimicrobial activity of extracts against Gram-positive and -negative bacteria was tested using the microdilution broth method. Extracts from single cultures had dose-dependent inhibitor activity against tested microorganisms, while co-culture extracts showed higher activity compared to extracts from monocultures. Furthermore, the antioxidant activity was investigated by 4 spectrophotometric methods. The highest antioxidant activity was obtained using the ferric reducing antioxidant power assay on algal monoculture and co-cultivation. In terms of anti-aging properties, inhibition of collagenase activity was recorded on S. rimosus monoculture and co-culture. Tested extracts showed no negative impact on the survival/development of zebrafish Danio rerio embryos, which taken together with other results implies their potential for broadening their biotechnological applications.