The impact of Rhizophagus intraradices (RI) interaction with phosphorus fertilization on the yield of Phaseolus vulgaris L. var. Negro Tacaná, and the N and P content in plant tissue and grain were determined. The experiment consisted of six treatments:1) Control, 2) RI, 3) 40 kg.ha-1 of P2O5, 4) RI + 40 kg.ha-1 of P2O5, 5) RI + 26 kg.ha-1 of P2O5, and 6) RI + 13 kg.ha-1 of P2O5, using a randomized block design with four replicates. Results probed that inoculation of plants with RI promoted a better health, growth and grain yield. In particular, grain yield inoculated with RI presented similar values as obtained with inorganic fertilization of 40 kg.ha-1 of P2O5, indicating that inorganic fertilization can be fully substituted by RI. The use of this technology provides an efficient use of soil nutrients, which is translated into a lower investment for producers.