Succinic acid production by immobilized cultures using spent sulphite liquor as fermentation medium
- Resource Type
- Authors
- Harris Papapostolou; Apostolis A. Koutinas; Lutgart Stragier; Willy Verstraete; Seraphim Papanikolaou; Maria Alexandri
- Source
- Bioresource Technology. 238:214-222
- Subject
- 0106 biological sciences
Environmental Engineering
Succinic Acid
Bioengineering
010501 environmental sciences
01 natural sciences
chemistry.chemical_compound
Bioreactors
010608 biotechnology
Carbon source
Sulfites
Sugar
Waste Management and Disposal
0105 earth and related environmental sciences
Chromatography
biology
Renewable Energy, Sustainability and the Environment
Actinobacillus
General Medicine
biology.organism_classification
Actinobacillus succinogenes
chemistry
Biochemistry
Succinic acid
Yield (chemistry)
Fermentation
Basfia succiniciproducens
- Language
- ISSN
- 0960-8524
Spent sulphite liquor (SSL) was used as carbon source for the production of succinic acid using immobilized cultures of Actinobacillus succinogenes and Basfia succiniciproducens on two different supports, delignified cellulosic material (DCM) and alginate beads. Fed-batch immobilized cultures with A. succinogenes in alginates resulted in higher sugar to succinic acid conversion yield (0.81g/g) than the respective yield achieved (0.65g/g) when DCM immobilized cultures were used. The final succinic acid concentration and yield achieved in fed-batch with immobilized cultures of B. succiniciproducens in alginates (45g/L and 0.66g/g) were higher than A. succinogenes immobilized cultures (35.4g/L and 0.61g/g) using nano-filtrated SSL as fermentation medium. Immobilized cultures of B. succiniciproducens in alginate beads were reused in four sequential fed-batch fermentations of nano-filtrated SSL leading to the production of 64.7g of succinic acid with a yield range of 0.42-0.67g/g and productivity range of 0.29-0.65g/L/h. The immobilized cultures improved the efficiency of succinic acid production as compared to free cell cultures.