IncRNA XIST Targets miR-124/JAG1 via CeRNA Mechanism to Facilitate the Migration and Proliferation of Tongue Squamous Cell Carcinoma
- Resource Type
- Authors
- Shuichao, Gao; Jianjun, Yu; Zhenfeng, Shan; Liang, Zuo; Weilun, Huang; Li, Gan; Hao, Tian
- Source
- Clinical Laboratory. 68
- Subject
- MicroRNAs
Tongue
Cell Line, Tumor
Carcinoma, Squamous Cell
Humans
RNA, Long Noncoding
Jagged-1 Protein
General Biochemistry, Genetics and Molecular Biology
Cell Proliferation
Tongue Neoplasms
- Language
- ISSN
- 1433-6510
This study was designed to characterize the effect of lncRNA XIST on the migration as well as proliferation of tongue squamous cell carcinoma (TSCC) and its associated molecular mechanisms.Chip-seq analysis screened the aberrant lncRNA in TSCC patients. CCK-8 and scratch experiment detected cell migration and proliferation in TSCC after lncRNA XIST inhibition. We predicted and verified lncRNA XIST target miRNA. CCK-8 and scratch test examined the cell migration and proliferation effects in TSCC after transfection of miR-124 mimics. Luciferase reporter experiment confirmed the interaction of miR-124 with JAG1. Western blot validated influence of lncRNA XIST on miR-124/JAG1 axis.We found that lncRNA XIST was up-regulated in TSCC patients, and it facilitated the TSCC cell migration and proliferation in vitro. lncRNA XIST regulated miR-124 expression through ceRNA mechanism. Up-regulating miR-124 significantly inhibited TSCC cell migration and proliferation. JAG1 acted as immediate target of miR-124. Moreover, lncRNA XIST targeted miR-124 to regulate JAG1 levels through the ceRNA mechanism.IncRNA XIST encourages TSCC migration and proliferation by modulating miR-124/JAG1 axis.