Development of enhanced sensitivity protocols on the RapidHIT™ 200 with a view to processing casework material
- Resource Type
- Authors
- Des Vanhinsbergh; David Shackleton; Simon Malsom; Lesley Ives; Jenny Pagram; Nicholas Andrews
- Source
- Science & Justice. 59:411-417
- Subject
- Protocol (science)
business.industry
Computer science
Sample (material)
010401 analytical chemistry
01 natural sciences
0104 chemical sciences
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Template
Enhanced sensitivity
030216 legal & forensic medicine
Sensitivity (control systems)
business
Computer hardware
- Language
- ISSN
- 1355-0306
The RapidHIT™ 200 device from IntegenX® provides a sample-to-profile platform that is capable of processing a variety of sample types. In this study we review the sensitivity of the 'Run Other' protocol for processing crime stain type samples containing various input quantities of DNA using the AmpFlSTR® NGMSElect™ Express PCR Amplification Kit cartridges available from IntegenX®. The range of DNA inputs which achieved useable results were not as desired and therefore various enhancements to the instruments extraction processes were investigated. These studies showed an improvement in the range of DNA input templates that could by processed on the RapidHIT™ 200 by using the enhanced methods and resulted in three new run protocols.