Selective ablation of biological tissue and single cells on a glass substrate by controlling the laser energy density of nanosecond 193 nm laser radiation
- Resource Type
- Authors
- Ranjith Karuparambil Ramachandran; Frank Vanhaecke; Thibaut Van Acker; Legna Colina-Vegas; Stijn Van Malderen
- Source
- Journal of Analytical Atomic Spectrometry. 34:1957-1964
- Subject
- Materials science
business.industry
Borosilicate glass
medicine.medical_treatment
Microscope slide
Substrate (electronics)
Radiation
Nanosecond
Laser
Ablation
Analytical Chemistry
law.invention
Wavelength
law
medicine
Optoelectronics
business
Spectroscopy
- Language
- ISSN
- 1364-5544
0267-9477
This paper describes the possibility of controlling and reducing the laser energy density of nanosecond 193 nm laser radiation in order to selectively ablate biological material from a glass substrate for LA-ICP-MS bioimaging applications. Atomic force microscopy and optical profilometry were used to study the shape of single-shot craters in dried gelatin droplets, ablated at low energy (