Additional file 1: Fig. S1. Multiple-sequence alignment of selected putative EBDH and AHY enzymes around the catalytic site of EBDH. Representative environmental Ahy enzymes were deduced from the gene sequences generated in this study. The conserved residues for catalytic functioning in EBDH are highlighted in red, whereas substitutions at these sites in putative AHY are shown in blue. Table S1. Primers designed in this study (len: length, pos: start position, gc: GC content; Tm: melting temperature). Table S2. Performance of primers