Open microfluidic gel electrophoresis: Rapid and low cost separation and analysis of DNA at the nanoliter scale
- Resource Type
- Authors
- Roland Zengerle; Peter Koltay; Laurent Tanguy; L. Riegger; Ludwig Gutzweiler; Tobias Gleichmann
- Source
- ELECTROPHORESIS. 38:1764-1770
- Subject
- 0301 basic medicine
Gel electrophoresis
chemistry.chemical_classification
Materials science
Fabrication
Capillary action
Clinical Biochemistry
Microfluidics
Evaporation
Nanotechnology
02 engineering and technology
Polymer
021001 nanoscience & nanotechnology
Chip
Biochemistry
Analytical Chemistry
03 medical and health sciences
Electrophoresis
030104 developmental biology
chemistry
0210 nano-technology
- Language
- ISSN
- 0173-0835
Gel electrophoresis is one of the most applied and standardized tools for separation and analysis of macromolecules and their fragments in academic research and in industry. In this work we present a novel approach for conducting on-demand electrophoretic separations of DNA molecules in open microfluidic (OM) systems on planar polymer substrates. The approach combines advantages of slab gel, capillary- & chip-based methods offering low consumable costs (