Determination of monofluoroacetate in powdered nutritional products by derivatization with 2-nitrophenylhydrazine and LC-MS/MS: First action 2015.04
- Resource Type
- Authors
- Reddy, Murali; Sullivan, Darryl; Alewijn, Martin; Austad, John; Boison, Joe; Christiansen, Scott; Cook, Jo Marie; De Vries, Jon; Indyk, Harvey; Joseph, George; Konings, Erik; Krynitsky, Alex; Phillips, Tom; Popping, Bert; Wong, John
- Source
- Journal of AOAC International 98 (2015) 4
Journal of AOAC International, 98(4), 1135-1139
- Subject
- Pharmacology
Chromatography
Chemistry
Extraction (chemistry)
Nutritional products
Analytical Chemistry
Dilution
chemistry.chemical_compound
BU Authenticiteit & Bioassays
BU Authenticity & Bioassays
Lc ms ms
Environmental Chemistry
Peptide bond
Protein precipitation
Life Science
Acetonitrile
Derivatization
Agronomy and Crop Science
Food Science
VLAG
- Language
- English
- ISSN
- 1060-3271
Determination of monofluoroacetate in powdered nutritional products by derivatization with 2-nitrophenylhydrazine and LC-MS/MS IS discussed. Samples are prepared by dilution in water followed by protein precipitation with acetonitrile. An aliquot of the sample extract is derivatized with 2-nitrophenylhydrazine (2-NPH) in the presence of 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). This reaction achieves the coupling of the carboxyl group of monofluoroacetate to the amino group of 2-NPH with the formation of an amide bond. Samples are processed through a solid-phase extraction (SPE) step in order to exchange.