A New Tool for Inducible Gene Expression in Caenorhabditis elegans
- Resource Type
- Authors
- Gabriela C. Monsalve; Keith R. Yamamoto; Jordan D. Ward
- Source
- Genetics, vol 211, iss 2
- Subject
- Transcriptional Activation
ligand-binding domain
Cell type
1.1 Normal biological development and functioning
medicine.medical_treatment
Glucocorticoid receptor
Investigations
Dexamethasone
Steroid
03 medical and health sciences
Receptors, Glucocorticoid
Glucocorticoid
0302 clinical medicine
Underpinning research
Receptors
Gene expression
Genetics
medicine
Animals
Humans
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Receptor
Glucocorticoids
Transcription factor
030304 developmental biology
0303 health sciences
biology
Forkhead Transcription Factors
biology.organism_classification
Ligand (biochemistry)
Cell biology
Genetic Techniques
drug inducible
gene expression
Generic health relevance
030217 neurology & neurosurgery
Biotechnology
Developmental Biology
- Language
- ISSN
- 1943-2631
Controlling protein activity and localization is a key tool in modern biology. Mammalian steroid receptor ligand-binding domain (LBD) fusions have been used in a range of organisms and cell types to inactivate proteins of interest until the cognate steroid ligand is applied. Here, we demonstrate that the glucocorticoid receptor LBD confers ligand-gated control of a heterologous gene expression system (Q system) and the DAF-16 transcription factor in Caenorhabditis elegans. These experiments provide a powerful tool for temporal control of protein activity, and will bolster existing tools used to modulate gene expression and protein activity in this animal.