Incarnatapeptins A and B, Nonribosomal Peptides Discovered Using Genome Mining and 1H/15N HSQC-TOCSY
- Resource Type
- Authors
- Kalindi D Morgan; David E. Williams; Marianne D. Sadar; Katherine S. Ryan; Carmen Adriana Banuelos; Marion Remigy; Raymond J. Andersen; Brian O. Patrick
- Source
- Organic Letters. 22:4053-4057
- Subject
- 010405 organic chemistry
Chemistry
Drug discovery
Organic Chemistry
In vitro cytotoxicity
Combined use
Computational biology
010402 general chemistry
01 natural sciences
Biochemistry
3. Good health
0104 chemical sciences
Chemical diversity
LNCaP
Screening method
Genome mining
Physical and Theoretical Chemistry
Heteronuclear single quantum coherence spectroscopy
- Language
- ISSN
- 1523-7052
1523-7060
Methods for the focused isolation of low-abundance natural products with specific chemical substructures could expand known bioactive chemical diversity for drug discovery. Here we report the combined use of genome mining and an 15N NMR-based screening method for the targeted isolation of the low-abundance piperazic-acid-containing peptides incarnatapeptins A (1) and B (3). Incarnatapeptin B (3) shows in vitro cytotoxicity to LNCaP prostate cancer cells.