Objective To study the parasitic fauna on serpentines under captive condition in zoological park of Tamilnadu, India. Methods Fecal samples were collected from ( n = 247) serpentines, Arignar Anna Zoological Park ( n = 22), Vandalur, Tamilnadu, India and Snake Park ( n = 27), Guindy, Tamilnadu, India and screened for endoparasites using sedimentation techniques. Ectoparasites were also reported in this study. Results Coprological examination ( n = 247) from captive snakes ( n = 49) on random analysis revealed strongyles were predominant in Arignar Anna Zoological Park, Vandalur and Snake Park, Guindy, however the parasites were absent in king cobras ( Ophiophagus hannah ). Eggs of Capillaria sp. showed less predominance in Vandalur and Gunidy. Rat snakes [ Ptyas mucosus ( P. mucosus )] showed higher prevalence of strongyle infection in Vandalur, and Russell's viper ( Daboia russelii ) showed higher prevalence in Guindy. Study on ectoparasites revealed Aponomma gerviasii ticks in P. mucosus , Indian cobras ( Naja naja ), king cobras ( Ophiophagus hannah ), reticulated pythons ( Python reticulates ) and Indian rock pythons ( Python molurus ), among them, the most heavy infestation was documented in P. mucosus ( n = 9). Conclusions Confinement favour stress and dysecdysis in captive condition affect the health status of snakes in zoological park.