Ribosome assembly in Escherichia coli strains lacking the RNA helicase DeaD/CsdA or DbpA
- Resource Type
- Authors
- Kai Virumäe; Lauri Peil; Jaanus Remme
- Source
- FEBS Journal. 275:3772-3782
- Subject
- 5.8S ribosomal RNA
Ribosome biogenesis
Cell Biology
Biology
Biochemistry
Ribosome
Molecular biology
Cell biology
Ribosome assembly
Large ribosomal subunit
Eukaryotic Small Ribosomal Subunit
Eukaryotic Ribosome
Molecular Biology
50S
- Language
- ISSN
- 1742-464X
Ribosome subunit assembly in bacteria is a fast and efficient process. Among the nonribosomal proteins involved in ribosome biogenesis are RNA helicases. We describe ribosome biogenesis in Escherichia coli strains lacking RNA helicase DeaD (CsdA) or DbpA. Ribosome large subunit assembly intermediate particles (40S) accumulate at 25 °C and at 37 °C in the absence of DeaD but not without DbpA. 23S rRNA is incompletely processed in the 40S and 50S particles of the DeaD− strain. Pulse labeling showed that the 40S particles are converted nearly completely into functional ribosomes. The rate of large ribosomal subunit assembly was reduced about four times in DeaD-deficient cells. Functional activity tests of the ribosomal particles demonstrated that the final step of 50S assembly, the activation step, was affected when DeaD was not present. The results are compatible with the model that predicts multiple DeaD-catalyzed structural transitions of the ribosome large subunit assembly.