Cytomegalovirus hyperimmunoglobulin: mechanisms in allo-immune response in vitro
- Resource Type
- Authors
- Wolfram Hoetzenecker; Stefan Hacker; F. Muehlbacher; T. Wliszczak; Andreas Pollreisz; Konrad Hoetzenecker; K. Sadeghi; Walter Klepetko; Hendrik Jan Ankersmit; E. Bielek; Andreas Mangold; M. Sachet
- Source
- European Journal of Clinical Investigation. 37:978-986
- Subject
- CD4-Positive T-Lymphocytes
Cellular immunity
CD3 Complex
T-Lymphocytes
Lymphocyte
Clinical Biochemistry
Cytomegalovirus
Immunoglobulins
Apoptosis
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Biochemistry
Natural killer cell
Interferon-gamma
Immune system
medicine
Humans
Antibody-dependent cell-mediated cytotoxicity
biology
Immunoglobulins, Intravenous
General Medicine
Interleukin-10
Killer Cells, Natural
Transplantation
Tolerance induction
medicine.anatomical_structure
Immunology
Leukocytes, Mononuclear
biology.protein
Interleukin-2
Lymphocyte Culture Test, Mixed
Antibody
- Language
- ISSN
- 1365-2362
0014-2972
Background Cytomegalovirus hyperimmunoglobulin (CMVIg) containing drugs are routinely administered in cardiac transplantation for prophylaxis against CMV disease. Yet little is known about their influence on transplant relevant immune functions. The aim of this study was to evaluate the effect of CMVIg on cellular immunity in in vitro experiments and to define their role in tolerance inducing mechanisms. Materials and methods/results CMVIg reduces proliferation in mixed lymphocyte reactions and anti-CD3 blastogenesis assays and is related to decreased production of immune modulating cytokines interleukin (IL)-2, interferonr (IFNgamma), IL-10. This antiproliferative effect is associated with a cell-cycle arrest in the G0/G1 phase and induction of apoptosis in CD8+ and natural killer cells. Co-incubation with CMVIg causes down-regulation of cell bound immunoglobulin and FcgammaRIII surface expression on natural killer cells and leads to attenuation of antibody dependent cellular cytotoxicity effector functions. Conclusions We conclude that CMVIg induces immunological features on leukocytes in vitro that are known to be related to tolerance induction. Our observations extend the current concept of CMVIg as passive CMV prophylaxis to a therapeutic drug compound capable of reducing allogeneic immune response.