High-speed polarization-resolved third-harmonic microscopy
- Resource Type
- Authors
- Arthur Boutillon; Joséphine Morizet; Guillaume Ducourthial; Chiara Stringari; Willy Supatto; Marie-Claire Schanne-Klein; Renaud Legouis; Emmanuel Beaurepaire
- Source
- Optica
Optica, 2019, 6 (3), pp.385-388. ⟨10.1364/OPTICA.6.000385⟩
Optica, Optical Society of America-OSA Publishing, 2019, 6 (3), pp.385-388. ⟨10.1364/OPTICA.6.000385⟩
- Subject
- [PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics]
Materials science
Microscope
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Polarimetry
01 natural sciences
law.invention
010309 optics
03 medical and health sciences
Optics
law
0103 physical sciences
Microscopy
Anisotropy
Circular polarization
030304 developmental biology
0303 health sciences
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics]
Birefringence
business.industry
Polarization (waves)
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging
Temporal resolution
business
- Language
- English
- ISSN
- 2334-2536
International audience; Polarization-resolved third-harmonic generation (P-THG) is a sensitive probe of material anisotropy and molecular ordering. Despite its promises, this property has little been used in biological tissues due to the lack of measurement schemes compatible with dynamic samples. We report here on the development of a fast P-THG microscope where excitation polarization is switched between line scans using an electro-optic modulator, providing temporal resolution in the 10 ms range for the polarimetric measurement. We demonstrate novel applications enabled by this approach, associated with Fourier-based analysis: probing molecular order in deforming lipid structures undergoing phase transition; revealing crystallinity of flowing particles in the zebrafish embryo’s inner ear; and detecting birefringence in vivo. These results establish that P-THG is ideally suited for probing lipid organization and mineralization in dynamic biological environments.