PDF file - 194K, Supplemental Figure S1. A. Immunofluorescence staining: ERCC1 antibody of Sigma HPA029773 (upper row) and Lab Vision 8F1 (middle row) showed clear nuclear staining. Both cytoplasmic and nuclear staining of ERCC1 was seen using Santa Cruz FL297 (lower row), resulting in a more diffuse staining pattern. B. Correlation coefficient between AQUA and H scores for 8F1 staining, 0.69. Scores are read for each spot (plotted on X and Y axis). Supplemental Figure S2. ERCC1 expression using the antibody HPA029773 was localized in the nucleus of non-keratinizing (A) and keratinizing (B) squamous cell carcinoma (SCC). Upper panel: high ERCC1 AQUA score. Lower panel: low ERCC1 AQUA score. Note the staining intensity of cytokeratin and the nuclear stain DAPI are the same in all specimens.