LncRNA NEAT1 promotes glioma cancer progression via regulation of miR-98-5p/BZW1
- Resource Type
- Authors
- Xi-rui Wang; Gang Li; Yabin Li; Zhihuang Zhao; Ruijian Zhang; Jinxing Shang
- Source
- Bioscience Reports
- Subject
- 0301 basic medicine
NEAT1
Biophysics
Mice, Nude
Cell Cycle Proteins
Biology
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
In vivo
Cell Line, Tumor
Glioma
medicine
Animals
Humans
Molecular Biology
Research Articles
Cancer
Cell Proliferation
Mice, Inbred BALB C
Gene knockdown
Brain Neoplasms
Cell growth
Paraspeckle
BZW1
Cell Biology
medicine.disease
Tumor Burden
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
Blot
miR-98-5p
MicroRNAs
030104 developmental biology
Cell culture
030220 oncology & carcinogenesis
Disease Progression
Cancer research
Female
RNA, Long Noncoding
Carcinogenesis
Signal Transduction
- Language
- ISSN
- 1573-4935
0144-8463
Background: Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas.Methods: Quantitative real-time PCR technology was used to determine the expression levels of relevant genes in tumor tissues and cell lines. The protein expression levels were validated by Western blotting. Cell counting kit-8 (CCK-8) and colony formation assays were used to test the cell proliferation ability. A luciferase reporter assay was used to determine the interactions of the genes. Tumor xenografts were used to detect the role of NEAT1 in gliomas in vivo.Results: We demonstrated that NEAT1 up-regulated glioma cells and negatively correlated with miR-98-5p in glioma tissues. A potential binding region between NEAT1 and miR-98-5p was confirmed by dual-luciferase assays. NEAT1 knockdown inhibited glioma cell proliferation. The inhibition of miR-98-5p rescued the knockdown of NEAT1 in glioma cells. Basic leucine zipper and W2 domain containing protein 1 (BZW1) was identified as a direct target of miR-98-5p. We also identified that BZW1 was positively correlated with NEAT1 in glioma tissues. NEAT1 knockdown inhibited glioma cell proliferation in vivo via miR-98-5p/BZW1.Conclusion: Our results suggest that NEAT1 plays an oncogenic function in glioma progression. Targeting NEAT1/miR-98-5p/BZW1 may be a novel therapeutic treatment approach for glioma patients.