Two Enhancers Regulate HoxB Genes Expression During Retinoic Acid-Induced Early Embryonic Stem Cells Differentiation Through Long-Range Chromatin Interactions
- Resource Type
- Authors
- Wange Lu; Lei Zhang; Jian Zheng; Xueyuan Zhao; Zhongfang Zhao; Man Liu; Wenbin Wang; Jinfang Bi; Guangsong Su; Jiandang Shi
- Source
- Stem Cells and Development.
- Subject
- 0301 basic medicine
Retinoic acid
Cell Biology
Hematology
Biology
Embryonic stem cell
Chromatin
Cell biology
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
chemistry
embryonic structures
Gene expression
CRISPR
Enhancer
Gene
030217 neurology & neurosurgery
Developmental Biology
- Language
- ISSN
- 1557-8534
1547-3287
Homeobox B cluster (HoxB) genes play important roles in retinoic acid (RA)-induced early embryonic stem cells (ESCs) differentiation. Knowledge of regulation network of HoxB is important to further unveil the mechanism of ESCs differentiation. In this study, we identified two enhancers that were activated by RA treatment and 4C data showed long-range interactions between HoxB genes and the two enhancers. CRISPR/Cas9-mediated individual or compound deletion of the two enhancers significantly inhibits HoxB gene expression, and transcriptome analysis revealed that RA-induced early ESCs differentiation was blocked in the enhancer KO cells. We propose new mechanism by which two enhancers regulate HoxB gene expression by different regulation modes during RA-induced early ESCs differentiation through long-range chromatin interactions.