Micro method for the gas chromatographic determination of serum theophylline utilizing an organic nitrogen sensitive detector
- Resource Type
- Authors
- Vidmantas A. Raisys; James D. Lowry; Lorna J. Williamson
- Source
- Journal of Chromatography B: Biomedical Sciences and Applications. 143:83-88
- Subject
- chemistry.chemical_compound
Chromatography
chemistry
Detector
Extraction (chemistry)
medicine
chemistry.chemical_element
Theophylline
General Chemistry
Derivatization
Nitrogen
medicine.drug
- Language
- ISSN
- 0378-4347
A gas chromatographic micro method utilizing an organic nitrogen sentitive detector for the determination of serum theophylline is described. The method incorporates 3-isobutyl-1-methylxanthine as the internal standard and involves extraction and off-column derivatization of theophylline and the internal standard to their pentyl derivatives. Using 50 μl of serum, concentrations of 1 μg/ml in serum can easily be measured. The method is linear up to 50 μg/ml and the precision of the method is 3.4% in the therapeutic range. No interferences from endogenous compounds or from drugs commonly co-administered with theophylline have been encountered.