Additional file 1: Fig. S1. Graphs from absolute value of pupariation time shown in Fig. 1. Fig. S2. Decrease in survival of GF larvae in poor protein diet is diminished when Imp-L2 is overexpressed using Imp-L2 Gal4. Fig. S3. Gut enterocyte-specific knockdown of Imp-L2 by Myo1A Gal4 using different RNAi line from that in Fig. 2 causes a significant decrease in the survival rate of GF larvae. Fig. S4. Lp fails to promote developmental rate of enterocyte Imp-L2-silenced larvae. Fig. S5. Lp fails to increase the survival rate of enterocyte Imp-L2-silenced larvae. Fig. S6. Imp-L2 mutation does not impair the expression of gut peptidase genes and activation of Imd pathway induced by Lp mono-association.