Evaluation of immunohistochemical expression of ghrelin-producing rectal cells in Wistar rats underwent to the cafeteria diet
- Resource Type
- Authors
- Daniella Serafin Couto Vieira; André Muller Teive; Humberto Fenner Lyra Junior; Angélica Luciana Nau; Tiago Rafael Onzi; Aline Bogoni Costa; Leonardo de Lucca Schiavon; Armando José d'Acampora
- Source
- Acta Cirurgica Brasileira, Volume: 28, Issue: 8, Pages: 614-618, Published: AUG 2013
Acta Cirurgica Brasileira, Vol 28, Iss 8, Pp 614-618 (2013)
Acta Cirúrgica Brasileira v.28 n.8 2013
Acta Cirúrgica Brasileira
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
- Subject
- Male
medicine.medical_specialty
Cell type
Time Factors
RD1-811
Rectum
Cell Count
Cafeteria
Reference Values
Internal medicine
Dietary Carbohydrates
medicine
Animals
Obesity
Rats, Wistar
biology
business.industry
digestive, oral, and skin physiology
Organ Size
Diet, High-fat
biology.organism_classification
Immunohistochemistry
Ghrelin
Diet
Rats
Endocrinology
medicine.anatomical_structure
Surgery
Analysis of variance
Colorectal Neoplasms
business
- Language
- ISSN
- 0102-8650
PURPOSE:To investigate the impact of cafeteria diet on ghrelin expression in rectal tissue and identify the morphologic cell type. METHODS:Twenty-four male Wistar rats were divided into four subgroups of six animals each: RC1 (rat chow 1) and CAF1 (cafeteria diet 1) for a period of 30 days; RC2 (rat chow 2) and CAF2 (cafeteria diet 2) for a period of 60 days. The animal and rectal weight, the number and the type of immunoreactive ghrelin cells were recorded and compared between the subgroups. The statistical study was established by ANOVA and Student's t test. RESULTS:There was no difference in the total of immunoreactive cells (p=0.685) between the subgroups nor between weight and presence or absence of ghrelin expression (p=0.993). All the immunoreactive cells identified were closed-type. CONCLUSION:The cafeteria diet did not have influence on the amount of immunoreactive rectal cells of ghrelin and only one type (closed-type) of immunoreactive cells was expressed in the rectum.