Additional file 7: of PI3Kα/δ inhibition promotes anti-tumor immunity through direct enhancement of effector CD8+ T-cell activity
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- Carnevalli, Larissa; Sinclair, Charles; Taylor, Molly; Gutierrez, Pablo; Langdon, Sophie; Coenen-Stass, Anna; Mooney, Lorraine; Hughes, Adina; Jarvis, Laura; Staniszewska, Anna; Crafter, Claire; Sidders, Ben; Hardaker, Elizabeth; Hudson, Kevin; Barry, Simon
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Figure S5. IL2 RNA and Bcl family RNA heatmap and protein levels. (A) Purified naïve CD8+ T-cells were rested for 3 days with AZD8835 at a maximum of 10 μM under stimulated conditions with CD3/CD28 beads. (B) Heatmap shows mRNA expression levels of anti-apoptotic factors on purified CD8+ T-cells treated as in A. (C) Histogram showing increased mean fluorescence intensity (MFI) levels of BCL2 protein in cell treated with 0.3 μM of AZD8835 and CAL-101. Data represent ≥2 experiments. (PDF 75 kb)