High performance liquid chromatography analysis of tetrahydrozoline hydrochloride in ophthalmic solution by silica column eluted with aqueous solvent mixtures
- Resource Type
- Authors
- Hsiu O. Ho; Kuo Ching Wen; Ming Chuan Huang; Ming Thau Sheu
- Source
- Journal of Food and Drug Analysis. 10
- Subject
- Pharmacology
0303 health sciences
Chromatography
Aqueous solution
030309 nutrition & dietetics
Calibration curve
Elution
010401 analytical chemistry
01 natural sciences
High-performance liquid chromatography
0104 chemical sciences
Solvent
03 medical and health sciences
chemistry.chemical_compound
chemistry
Methanol
Triethylamine
Food Science
Tetrahydrozoline Hydrochloride
- Language
- ISSN
- 2224-6614
A HPLC method using bared silica column eluted with aqueous solvent mobile phase was developed for determination of tetrahydrozoline hydrochloride in ophthalmic preparations. A mixture of methanol and water (70:30, v/v) containing 0.03% triethylamine an d 0.02% acetic acid was used as mobile phase, and chlorpheniramine maleate as an internal standard. The flow rate was 1 mL/min and the detection was at 254 nm. The quantitation limit was 1.0 µg/mL. Average recoveries range from 98.9 to 99.9%. The linearity of the calibration curve of tetrahydrozoline hydrochloride was well correlated (r 2 = 0.9996) within the range from 12.5 to 500 µg/mL as well as from 1.0 to 20 µg/mL (r 2 = 0.9997). This study further reports a simple and quick method for routine quantitative analysis of tetrahydrozoline hydrochlo ride in an ophthalmic solution that contains a relatively high concentration of sulfamethoxazole sodium and methyl paraben.