Correlation of Immunomodulatory Cytokine Expression with Histopathological Changes and Viral Antigen in a Hamster Model of Equine Herpesvirus-9 Encephalitis
- Resource Type
- Authors
- Shehata Anwar; Hiadeto Fukushi; Tokuma Yanai; Osama Abas; Abdel-Rhman Abou-Rawash; Hoda A. Abd-Ellatieff
- Source
- Journal of Comparative Pathology. 180:46-54
- Subject
- 040301 veterinary sciences
Hamster
Biology
medicine.disease_cause
030308 mycology & parasitology
Pathology and Forensic Medicine
Proinflammatory cytokine
0403 veterinary science
03 medical and health sciences
Virus antigen
Downregulation and upregulation
Cricetinae
medicine
Animals
Varicellovirus
Horses
Antigens, Viral
Equine herpesvirus 9
0303 health sciences
General Veterinary
Brain
Meningoencephalitis
04 agricultural and veterinary sciences
medicine.disease
Disease Models, Animal
Immunology
Cytokines
Encephalitis
Immunohistochemistry
Horse Diseases
- Language
- ISSN
- 0021-9975
A group of hamsters (n = 25) was intranasally infected with equine herpesvirus-9 (EHV-9) and mRNA transcription levels of several proinflammatory (IFN-γ, TNF-α and IL-6) and anti-inflammatory (IL-4, IL-10 and TGF-β) cytokines were investigated in brain tissue using RT-qPCR. These levels were correlated with the severity of sequential histopathological changes and intensity of immunohistochemical labelling of virus antigen in brain. Early and progressive upregulation of all the proinflammatory and anti-inflammatory cytokines investigated (P 0.05) was correlated with increasing severity of encephalitis and viral antigen expression from 2 days post infection (dpi) with a peak at 4-5 dpi (P 0.05).