Production and analysis of specific monoclonal antibodies against the cell wall of Mycobacterium avium
- Resource Type
- Authors
- H Mauch; U Klause; S Wagner
- Source
- Medical microbiology and immunology. 181(1)
- Subject
- Microbiology (medical)
medicine.drug_class
Immunology
Cell
Immunoblotting
Dot blot
Enzyme-Linked Immunosorbent Assay
Cross Reactions
Monoclonal antibody
Epitope
Microbiology
Immunophenotyping
Epitopes
Mice
Antigen
Antibody Specificity
medicine
Immunology and Allergy
Animals
Mice, Inbred BALB C
biology
Molecular mass
Antibodies, Monoclonal
General Medicine
Molecular biology
Antibodies, Bacterial
Molecular Weight
medicine.anatomical_structure
Cytoplasm
biology.protein
Female
Antibody
Bacterial Outer Membrane Proteins
Mycobacterium avium
- Language
- ISSN
- 0300-8584
Monoclonal antibodies (mAbs) against Mycobacterium avium were produced which specifically reacted with cell walls of M. avium. The binding pattern was not limited to one subtype. The three most specific mAbs showed binding to the outer surface of M. avium but not to other mycobacterial or bacterial cell surfaces. The combined results of enzyme-linked immunosorbent assay, immunoblot and dot blot showed that mAb 4A006 bound to an epitope located in the cell wall and on the cell surface and mAb 4A010 to an epitope exposed on the cell surface and the cytoplasm. The mAb 4A009-binding epitope was only detectable on the cell surface but not in the cell wall or cytoplasmic fractions of M. avium. In the immunoblot technic a protein antigen with a molecular mass of 27-29 kDa was identified by the mAbs. The mAb 4A006 reacted with 142 out of 143 M. avium subtypes 1, 4 and 8 obtained from AIDS patients. These mAbs seem to be applicable for the identification of M. avium complex after culture.