Fibroblast growth factor receptor signalling has a role in lobuloalveolar development of the mammary gland
- Resource Type
- Authors
- D. Jackson; T. Crafton; Richard Poulsom; Clive Dickson; I. Rosewell; J. Bresnick; G. Stamp
- Source
- Journal of Cell Science. 110:1261-1268
- Subject
- Genetically modified mouse
medicine.medical_specialty
animal structures
Mammary gland
Gene Expression
Mice, Transgenic
Biology
Fibroblast growth factor
Mice
Mammary Glands, Animal
Pregnancy
Internal medicine
medicine
Animals
RNA, Messenger
Receptor, Fibroblast Growth Factor, Type 1
Transgenes
Receptor, Fibroblast Growth Factor, Type 2
Promoter Regions, Genetic
Kinase
Mouse mammary tumor virus
Caseins
Proteins
Receptor Protein-Tyrosine Kinases
Cell Biology
Milk Proteins
biology.organism_classification
Receptors, Fibroblast Growth Factor
Phenotype
Cell biology
Fibroblast Growth Factors
medicine.anatomical_structure
Endocrinology
Mammary Tumor Virus, Mouse
Mammary Epithelium
Fibroblast growth factor receptor
RNA
Female
Signal Transduction
- Language
- ISSN
- 1477-9137
0021-9533
We have used the mouse mammary tumor virus promoter to express two dominant negative (DN) fibroblast growth factor receptor (FGFR) isoforms in the mammary epithelium of transgenic mice. While expression of DN-FGFR1(IIIc) showed no discernible phenotype, a similar kinase negative form of FGFR2(IIIb) caused a marked impairment of lobuloalveolar development. The growth retardation was apparent by mid-pregnancy and persisted in the post-partum glands. Despite the substantial underdevelopment of the mammary gland there was a measurable lactational response, but it was insufficient to properly sustain the new-born pups. These findings demonstrate that fibroblast growth factor signalling is necessary for pregnancy dependent lobuloalveolar development of the mammary gland.