Comparison of the HPLC-separated species patterns of phosphatidic acid, CDP-diacylglycerol and diacylglycerol synthesized de novo in rat liver microsomes (a new method)
- Resource Type
- Authors
- Keizo Waku; D. Kunze; Bernd Rüstow; Harald Rabe; Yasuhito Nakagawa; G. Reichmann
- Source
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 961:364-369
- Subject
- Male
Cytidylyltransferase
Phosphatidate Phosphatase
Biophysics
Phosphatidic Acids
Biology
Biochemistry
Glycerides
Diglycerides
Acylation
chemistry.chemical_compound
Endocrinology
Biosynthesis
Methods
Animals
Diglyceride
Chromatography, High Pressure Liquid
Diacylglycerol kinase
Cytidine Diphosphate Diglycerides
Chromatography
Nucleoside Diphosphate Sugars
Substrate (chemistry)
Phosphatidic acid
Nucleotidyltransferases
Rats
carbohydrates (lipids)
chemistry
Microsomes, Liver
Microsome
lipids (amino acids, peptides, and proteins)
- Language
- ISSN
- 0005-2760
The species pattern of phosphatidic acid was compared with that of CDP-diacylglycerol and diacylglycerol synthesized de novo by glycerol 3-phosphate acylation in a CoA ester-generating system in liver microsomes. The similarity of the species patterns of phosphatidic acid and CDP-diacylglycerol indicated that the CTP-phosphatidyl cytidylyltransferase showed no selectivity for individual species of its phosphatidic acid substrate. Since the species pattern of diacylglycerol deviated from that of phosphatidic acid, a slight acyl selectivity of the phosphatidic acid phosphohydrolase or a slight inhomogeneity of its substrate pool might be assumed. For the determination of the molecular species of CDP-diacylglycerol, a new method was developed. By incubation of CDP-diacylglycerol with oligonucleate 5'-nucleotidohydrolase (phosphodiesterase), phosphatidic acid was produced. The CDP-diacylglycerol-derived phosphatidic acid was methylated with diazomethane and then separated by reverse-phase HPLC in 15 molecular species.