A high-throughput multiplex genetic detection system for Helicobacter pylori identification, virulence and resistance analysis
- Resource Type
- Authors
- Yong Wu; Michal A. Olszewski; Xu Lingli; Yi Fang; Fuju Zhao; Hu Zhao; Shiwen Wang; Yang Changqing; Yingxin Miao; Hu Binjie; Mimi Kong; Bian Haipeng; Yanmei Zhang
- Source
- Future Microbiology. 11:1261-1278
- Subject
- Male
0301 basic medicine
Gastrointestinal Diseases
Biopsy
DNA Mutational Analysis
Antibiotics
Drug resistance
0302 clinical medicine
RNA, Ribosomal, 16S
Genotype
Multiplex
Aged, 80 and over
High-Throughput Nucleotide Sequencing
Middle Aged
Anti-Bacterial Agents
RNA, Ribosomal, 23S
Female
030211 gastroenterology & hepatology
medicine.drug
Adult
DNA, Bacterial
Microbiology (medical)
China
Peptic Ulcer
Virulence Factors
medicine.drug_class
030106 microbiology
Virulence
Microbial Sensitivity Tests
Biology
Microbiology
Helicobacter Infections
Young Adult
03 medical and health sciences
Metronidazole
Drug Resistance, Bacterial
medicine
Humans
Aged
Base Sequence
Helicobacter pylori
Amoxicillin
biology.organism_classification
Virology
Gastrointestinal Tract
Genes, Bacterial
Multiplex Polymerase Chain Reaction
- Language
- ISSN
- 1746-0921
1746-0913
Aim: We established a high-throughput multiplex genetic detection system (HMGS) for identification of Helicobacter pylori with concomitant analysis of virulence and drug resistance. Materials & methods: Confirmed 132 H. pylori cultures from gastric biopsies were screened by 20-gene site-HMGS, sequencing and E-test. Results: HMGS was highly sensitive and specific for H. pylori identification. Concordance rate between HMGS and sequencing averaged 94.5% (virulence genes) and 97.3% (resistance genes). Observed resistance rates to four mainstream antibiotics were high, except for amoxicillin. Significant association between virulence genotype and risks for specific gastrointestinal diseases was found for five genes. Metronidazole resistance in peptic ulcer patients was significantly higher. Conclusion: HMGS is an effective method for H. pylori identification and analysis of virulence and drug resistance.