International audience; Within the marine realm, diatoms play a major role in trophic food web and carbon export. On the other hand, Zooplankton grazing pressure on diatoms may contribute to carbon export through faecal pellets emission in the surface mixed layer or after vertical migration in the mesopelagic layers. Change in the water column stratification induced by global warming should increase the nutrients limitation in the surface ocean. Nitrogen and Silicon limitation during two diatoms growth monospecific culture (Chaetoceros neogracile and Skeletonema marinoi) conducted to a quick modulation of the diatom Si/C ratio and cell chain length of S. marinoi. To assess the influence of zooplankton, grazing incubation with both diatoms and copepods has been performed. Change in Si/C ratio and cell chain length influenced the ingestion rate of three copepods species (Acartia clausi, Calanus helgolandicus and Euterpina acutifrons) and faecal pellets emission and composition. The influence nutrient limitations during diatoms growth on copepods grazing could modulate biological pump of carbon through an increase of aggregates formation within the surface water and export more particles.