Gender is one of the main factors explored in earlier thermal comfort studies. The main objective of this work is to analyse potential gender effects on thermal perception under equivalent thermal conditions. The method is based on controlled experiments involving human subjects who were exposed to different thermal conditions in a climate chamber. During the experiments, participants were asked to answer questionnaires including background questions (e.g. physical characteristics) and thermal perception questions (thermal sensation, preference, comfort and acceptability). The criteria for the subject’s participation took into account age, gender, body composition, health condition and thermal history. In total, 24 subjects participated in the experiments (50% females). Results showed that, in fact, gender had no significant effect on thermal perception under equivalent thermal conditions.