The hepato-protective effect of H2S-modified and non-modified mesenchymal stem cell exosomes on liver ischemia-reperfusion injury in mice: The role of MALAT1
- Resource Type
- Authors
- Maryam J. Sameri; Feryal Savari; Khojasteh Hoseinynejad; Amir Danyaei; Seyed Ali Mard
- Source
- Biochemical and biophysical research communications. 635
- Subject
- Male
Biophysics
Mesenchymal Stem Cells
Cell Biology
Exosomes
Biochemistry
Mice
Liver
Ischemia
Reperfusion Injury
Animals
Humans
RNA, Long Noncoding
Molecular Biology
- Language
- ISSN
- 1090-2104
Ischemia-reperfusion injury (IRI) by causing histopathological changes is considered one of the most important causes of liver failure and dysfunction after surgery which affect graft outcomes. Stem cells are new promising approaches to treating different diseases. One of the critical strategies to improve their function is the preconditioning of their culture medium. This study compared the effect of NaHS-modified and non-modified mesenchymal stem cell exosomes on liver ischemia-reperfusion injury in mice.Human umbilical cord-derived MSC (MSC) cultured in a 75 cmAdministration of both MSC-Exo and H2S-Exo improved liver function by reducing inflammatory cytokines, cellular apoptosis, liver levels of total oxidant status, and liver aminotransferases. The results showed that protecting effect of MSC exosomes enhanced following NaHS preconditioning of cell culture medium.MSC-Exo and H2S-Exo had hepato-protective effects against injuries induced by ischemia-reperfusion in mice. NaHS preconditioning of mesenchymal stem cells could enhance the therapeutic effects of MSC-derived exosomes.