This paper reports a novel method to synthesize magnetic, stimuli-sensitive latex nanoparticles made with magnetite/poly(N-isopropylacrylamide-co-acrylic acid) (Fe 3 O 4 /P(NIPAAm-co-AAc)). To form a stabilized suspended core, iron oxide (Fe 3 O 4 ) was functionalized with AAc such that further polymerization with NIPAAm and AAc monomers could occur. The P(NIPAAm-co-AAc) shell layer exhibited thermosensitive properties. The inclusion of Fe 3 O 4 into the latex nanoparticles was confirmed using transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction spectroscopy, thermogravimetric analyzer (TGA), and superconducting quantum interference device magnetometer. The NIP–(AAc2.6–Fe) latex nanoparticles contained 2.25% Fe 3 O 4 (by weight), as determined by TGA analysis. The particle diameters measured approximately 160–240 nm with a lower critical solution temperature of 35 °C. These novel magnetic stimuli-responsive latex nanoparticles have potential applications in numerous fields, such as catalyst supports, protein immobilization, cancer therapy, target drug delivery systems, and other biomedical applications.