Detection of tumour lymphovascular space invasion using dual cytokeratin and CD31 immunohistochemistry
- Resource Type
- Authors
- Ian Jacobs; Ayse Ayhan; Naveena Singh; George D. Wilbanks; Farhad Alexander-Sefre; H B Salveson
- Source
- Journal of clinical pathology. 56(10)
- Subject
- Pathology
medicine.medical_specialty
Haematoxylin
Biology
Sensitivity and Specificity
Pathology and Forensic Medicine
Metastasis
Lymphatic System
chemistry.chemical_compound
Cytokeratin
Short Reports
Predictive Value of Tests
medicine
Carcinoma
Biomarkers, Tumor
Humans
Neoplasm Invasiveness
Hematoxylin
Staining and Labeling
Endometrial cancer
General Medicine
medicine.disease
Immunohistochemistry
Lymphovascular
Staining
Endometrial Neoplasms
Platelet Endothelial Cell Adhesion Molecule-1
chemistry
Adenocarcinoma
Eosine Yellowish-(YS)
Keratins
Female
- Language
- ISSN
- 0021-9746
Background: Lymphovascular space invasion (LVSI) is an important step in the complex process of tumour metastasis. Various methods have been used in the past to improve the histological detection of LVSI. Aims: To develop a sensitive immunohistochemical method for the detection of LVSI. Methods: Paraffin wax blocks from 108 patients who had undergone hysterectomy for stage I endometrial cancer were retrieved. Dual immunostaining for pancytokeratin and the CD31 endothelial cell marker was carried out on 4 μm sections cut from these bocks and compared with conventional haematoxylin and eosin staining. Results: The detection rate for LVSI increased threefold compared with conventional haematoxylin and eosin staining in the test group. Conclusion: This finding suggests that LVSI is a much more common phenomenon than previously thought and questions current understanding of tumour metastasis.