The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication
- Resource Type
- Authors
- Anne Aublin-Gex; Nathalie Davoust; Vincent Lotteau; Alessia Ruggieri; Thibault Chantier; Ralf Bartenschlager; Benoît de Chassey; Laurène Meyniel-Schicklin; Lionel Tafforeau; Patrice Andre; Fabrine Pradezynski; Philippe E. Mangeot; Laure Perrin-Cocon; Claire Ciancia
- Source
- P L o S Pathogens, 9 (7
PLoS Pathogens, Vol 9, Iss 7, p e1003440 (2013)
PLoS Pathogens
- Subject
- Proteomics
Adenosine Deaminase
viruses
Dengue virus
Viral Nonstructural Proteins
medicine.disease_cause
Virus Replication
Influenza A Virus, H1N1 Subtype
lcsh:QH301-705.5
Genetics
Systems Biology
Serine Endopeptidases
RNA-Binding Proteins
virus diseases
Recombinant Proteins
Host-Pathogen Interaction
Ribonucleoproteins
Influenza A virus
Helper virus
Host-Pathogen Interactions
Biologie
Research Article
lcsh:Immunologic diseases. Allergy
Virulence Factors
Immunology
Respiratory Mucosa
Biology
Microbiology
Cell Line
VP40
Species Specificity
Viral entry
Virology
Two-Hybrid System Techniques
Influenza, Human
Viral structural protein
medicine
Viroplasm
Humans
Protein Interactions
Molecular Biology
Computational Biology
Biological Transport
Dengue Virus
biochemical phenomena, metabolism, and nutrition
Viral Replication
NS2-3 protease
Viral replication
lcsh:Biology (General)
Parasitology
lcsh:RC581-607
- Language
- English
Influenza A NS1 and NS2 proteins are encoded by the RNA segment 8 of the viral genome. NS1 is a multifunctional protein and a virulence factor while NS2 is involved in nuclear export of viral ribonucleoprotein complexes. A yeast two-hybrid screening strategy was used to identify host factors supporting NS1 and NS2 functions. More than 560 interactions between 79 cellular proteins and NS1 and NS2 proteins from 9 different influenza virus strains have been identified. These interacting proteins are potentially involved in each step of the infectious process and their contribution to viral replication was tested by RNA interference. Validation of the relevance of these host cell proteins for the viral replication cycle revealed that 7 of the 79 NS1 and/or NS2-interacting proteins positively or negatively controlled virus replication. One of the main factors targeted by NS1 of all virus strains was double-stranded RNA binding domain protein family. In particular, adenosine deaminase acting on RNA 1 (ADAR1) appeared as a pro-viral host factor whose expression is necessary for optimal viral protein synthesis and replication. Surprisingly, ADAR1 also appeared as a pro-viral host factor for dengue virus replication and directly interacted with the viral NS3 protein. ADAR1 editing activity was enhanced by both viruses through dengue virus NS3 and influenza virus NS1 proteins, suggesting a similar virus-host co-evolution.
Journal Article
info:eu-repo/semantics/published