Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
- Resource Type
- Authors
- Berardi, Massimiliano; Bielawski, Kevin; Rijnveld, Niek; Gruca, Grzegorz; Aardema, Hilde; van Tol, Leni; Wuite, Gijs; Akca, B Imran; FAH klinische reproductie; dES/dFAH FR
- Source
- Communications Biology
Communications biology, 4(1):610, 1-7. Nature Research
Communications Biology, 4(1), 1. Springer Nature
Berardi, M, Bielawski, K, Rijnveld, N, Gruca, G, Aardema, H, van Tol, L, Wuite, G & Akca, B I 2021, ' Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution ', Communications biology, vol. 4, no. 1, 610, pp. 1-7 . https://doi.org/10.1038/s42003-021-02121-1
Communications Biology, Vol 4, Iss 1, Pp 1-7 (2021)
- Subject
- Optics and Photonics
Materials science
QH301-705.5
Medicine (miscellaneous)
Biocompatible Materials
02 engineering and technology
Biochemistry
Models, Biological
Article
General Biochemistry, Genetics and Molecular Biology
Displacement (vector)
03 medical and health sciences
Mechanobiology
Optics
Materials Testing
Animals
Nanotechnology
Biology (General)
Nanoscopic scale
Image resolution
030304 developmental biology
0303 health sciences
Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
business.industry
Biological techniques
Resolution (electron density)
High-throughput screening
Pipette
021001 nanoscience & nanotechnology
Elasticity
Biomechanical Phenomena
Interferometry
Oocytes
Cattle
Nanometre
Stress, Mechanical
0210 nano-technology
General Agricultural and Biological Sciences
business
SDG 6 - Clean Water and Sanitation
Genetics and Molecular Biology(all)
- Language
- English
- ISSN
- 2399-3642
Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.
Beradi et al. establish a micropipette aspiration platform which enables sensitive real-time viscoelastic measurements of soft biomaterials. They use it to characterize the rheological behavior of cells and soft materials at very small deformations, yielding consistent and reproducible values.