Lukulo upstream watershed is one of the watersheds in Central Java Province, which passes through 3 districts; Kebumen, Wonosobo, and Banjarnegara. Lukulo upstream watershed has eight sub-watersheds located in areas with diverse topography and lithology. The diversity of topography and lithology affects the characteristics of the area, as does the physical condition of the soil. The physical condition of this soil affects the runoff. Soil’s physical properties control rainwater to run off and have important effects on runoff behavior in watersheds. This study aims to determine the physical properties of the soil against runoff in each sub-watershed and each part of the watershed. The method used uses a laboratory, studio, and field approach. The results obtained show that the physical properties of the soil in the Lukulo upstream watershed affect the amount of runoff resistance in the upstream area; in the downstream area the runoff is relatively good. The study also shows two different results. In the Loning watershed, the velocity of downstream runoff is higher than the upstream, while in the Lokidang and Medana watershed, level of surface water flow is lower in the downstream area.