Fluorine is a component of atmospheric emissions in industrial areas. It negatively affects plant development and weakens the defense systems, thus making plants vulnerable to extreme environmental conditions. The heat shock proteins (HSP) are known to promote the plant resistance to various biotic and abiotic stresses. We studied the action of sodium fluoride (NaF) on growth, viability, respiration, transmembrane electric potential at the inner mitochondrial membrane (mtΔΨ), the development of induced thermotolerance, and HSP synthesis in the cell culture of Arabidopsis thaliana (L) Heynh (accession Columbia). The treatment with 20 mM NaF (for 120 min) had no negative influence on viability of the cell culture but inhibited the development of induced thermotolerance and suppressed the induction of HSP (Hsp101 and Hsp17.6) synthesis during mild heat stress (37°C). At the same time, the treatment with NaF inhibited respiration and suppressed the increase in mtΔΨ induced by mild heat stress. Hence, the negative impact of NaF on plants might arise from its ability to inhibit synthesis of stress proteins indispensible for plant adaptation to changing environmental conditions.