Organic production represents a process of sustainable development of the rural areas in accordance with the available resources and tradition, also implies comprehensive crop and livestock production, which ensures the preservation and restoration of natural resources, strongly supports return to the traditional values and knowledges. The new Strategy for Agriculture and Rural development in Serbia from 2014-2024 defines goals and priorities for further development of agriculture. One of priority topics is the promotion of organic production. Sjenica-Pester plateau belongs to High Nature Value region of Serbia and is well known for its rich biodiversity with favorable floristical composition of pastures and meadows. Organic sheep production is a perspective opportunity for rural regional development. Sjenica sheep is one of the most popular transboundry Zackel type, traditionally reared in the highly mountain regions of Sebia, mainly in the Sjenica Pester-plateu, where it was originally developed. The finding of favorable content of CLA and n-6:n-3 ratio, contribute to the advanced phenotypic characterization of Sjenica Zackel sheep type, the determination of locally adapted breed value and in making a realistic decision for the promotion of sustainable use of Sjenica Zackel sheep type. Proceedings