S1. Seahorse assays on C4-2B and PC3 cells cultured in 2D. S2. Seahorse assays on C4-2B and PC3 cells cultured in 2D and treated with IACS-10759. S3. Luciferase assay and crystal violet staining are comparable methods for monitoring PCa cells growth. S4. Representative pictures of tumor-free, PC3 tumor-bearing control and PC3 tumor-bearing IACS-10759 treated tibiae.