Expression and Presence of OPG and RANKL mRNA and Protein in Human Periodontal Ligament with Orthodontic Force
- Resource Type
- Authors
- Dabeiba Adriana García; Liliana Otero; Liseth Wilches-Buitrago
- Source
- Gene Regulation and Systems Biology, Vol 10 (2016)
Gene Regulation and Systems Biology
Gene Regulation and Systems Biology, Vol 2016, Iss 10, Pp 15-20 (2016)
- Subject
- 0301 basic medicine
musculoskeletal diseases
medicine.medical_specialty
Pathology
03 medical and health sciences
0302 clinical medicine
Osteoprotegerin
Internal medicine
Genetics
medicine
Periodontal fiber
bone modeling
orthodontic forces
Receptor
Molecular Biology
lcsh:QH301-705.5
Ecology, Evolution, Behavior and Systematics
Original Research
Messenger RNA
biology
business.industry
Activator (genetics)
RANKL
030206 dentistry
Computer Science Applications
Reverse transcription polymerase chain reaction
030104 developmental biology
Endocrinology
lcsh:Biology (General)
biology.protein
OPG
Analysis of variance
business
- Language
- English
- ISSN
- 1177-6250
Objective The objective of this study is to investigate the expression and concentration of ligand receptor activator of NFκB (RANKL) and osteoprotegerin (OPG) in human periodontal ligament (hPDL) with orthodontic forces of different magnitudes. Methods Right premolars in 32 patients were loaded with 4oz or 7oz of orthodontic force for 7 days. Left first premolars were not loaded. After 7 days, premolars were extracted for treatment as indicated. OPG and RANKL mRNA expressions were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and ELISA was used to assess OPG and RANKL protein concentration in compression and tension sides of PDL. Data were subjected to analysis of variance and Tukey tests. Results There was statistically significant difference in RANKL concentration on comparing control teeth with tension and compression sides of the experimental teeth ( P < 0.0001). The expression of mRNA RANKL was increased in the tension and compression sides with 4oz ( P < 0.0001). OPG did not show statistically significant association with any group. Changes in RANKL/OPG protein ratio in experimental and control groups showed statistically significant difference ( P < 0.0001). Conclusions RANKL protein levels are elevated in hPDL loaded with orthodontic forces, suggesting that RANKL protein contributes to bone modeling in response to the initial placement of orthodontic force.