The R27080 glycoprotein is abundantly secreted from human cytomegalovirus-infected fibroblasts
- Resource Type
- Authors
- M J Gerhart; J Müllberg; C T Rauch; A Kaykas; M L Hsu; D Cosman
- Source
- The Journal of general virology. 80
- Subject
- Human cytomegalovirus
animal structures
Glycosylation
Viral protein
viruses
Molecular Sequence Data
Cytomegalovirus
Biology
medicine.disease_cause
Transfection
chemistry.chemical_compound
Open Reading Frames
Viral Proteins
Virology
medicine
Animals
Humans
Amino Acid Sequence
Peptide sequence
Cells, Cultured
Glycoproteins
chemistry.chemical_classification
Methionine
virus diseases
medicine.disease
Molecular biology
Open reading frame
chemistry
COS Cells
Glycoprotein
Protein Processing, Post-Translational
Cysteine
- Language
- ISSN
- 0022-1317
A 45 kDa glycoprotein was purified from the culture media of human cytomegalovirus (HCMV)-infected fibroblasts. N-terminal sequencing revealed that the protein, R27080, is the translation product of the R27080 open reading frame of HCMV. R27080 is highly glycosylated and contains no cysteine or methionine residues. Proteolytic cleavage of R27080 by a furin-like enzyme was analysed in transfected COS-7 cells. R27080 is the first identified viral protein secreted from HCMV-infected cells.