Figure S5. Despite inter-replicate variability, cultures can be clustered and accurately classified. (a) Representative images of both hippocampal and cortical cultures at different DIV for 3 different replicates; (b) PCA based on different descriptor sets for individual replicates. PCA based on the integrated descriptor set resulted in the best clustering (nbio = 3 x ntech = 6) (Ellipses show the 67% confidence interval); (c) Confusion matrices of classification results using a random forest. The misclassification rate (MCR) was lowest using the integrated morphological descriptor set (nbio = 3 x ntech = 6); (d) Confusion matrices of classification results using a linear discriminant analysis. The MCR, compared to RFC, was lower for morphological data, but higher for functional data (nbio = 3 x ntech = 6). (PDF 10993 kb)