How to draw the line - Raman spectroscopy as a tool for the assessment of biomedicines
- Resource Type
- Authors
- Walter Matheis; Christel Kamp; Isabelle Bekeredjian-Ding; Björn Becker; Volker Öppling
- Source
- Biological chemistry, 402(8):1001-1006
- Subject
- Quality Control
Computer science
010401 analytical chemistry
Clinical Biochemistry
standardisation
machine learning
quality control
Raman spectroscopy
pre-processing
Biomedizinische Technik
vaccine
02 engineering and technology
021001 nanoscience & nanotechnology
Spectrum Analysis, Raman
01 natural sciences
Biochemistry
0104 chemical sciences
Open source data
Systems engineering
0210 nano-technology
Molecular Biology
- Language
- ISSN
- 1437-4315
Biomedicines are complex biochemical formulations with multiple components that require extensive quality control during manufacturing and in subsequent batch testing. A proof-of-concept study has shown that an application of Raman spectroscopy can be beneficial for a classification of vaccines. However, the complexity of biomedicines introduces new challenges to spectroscopic methodology that require advanced experimental protocols. We further show the impact of analytical protocols on vaccine classification using R as an Open Source data analysis platform. In conclusion, we advocate for standardized and transparent experimental and analytical procedures and discuss current findings and open challenges.