Hybrid biomimetic assembly enzymes based on ZIF-8 as 'intracellular scavenger' mitigating neuronal damage caused by oxidative stress
- Resource Type
- Authors
- Li, Qing; Feng, Ruixia; Chang, Zhaohui; Liu, Xiaojun; Tang, Hao; Bai, Qian
- Source
- Frontiers in Bioengineering and Biotechnology. 10
- Subject
- Histology
Biomedical Engineering
Bioengineering
Biotechnology
- Language
- ISSN
- 2296-4185
Superoxide dismutase (SOD) was immobilized in zeolite imidazolate framework-8 (ZIF-8) through biomimetic mineralization method, namely SOD@ZIF-8, which was then used in the treatment of nerve damage by eliminating reactive oxygen species (ROS). A series of chemical characterization and enzymatic activity researches revealed that SOD was successfully embedded into ZIF-8 without apparent influence on the antioxidant activity of SOD. Cell level experiments showed that SOD@ZIF-8 could be effectively endocytosed by cells. The activity of SOD@ZIF-8 in scavenging ROS played a critical role in protecting SHSY-5Y cells from MPP+-induced cell model and relieving cell apoptosis, indicating that SOD@ZIF-8 could effectively rescue ROS-mediated neurological disorders though removing excessive ROS produced in vitro.