Additional file 1. Table S1. Summary of simulated variants. VAFs were binned into 0.01, expressed as lower limit (inclusive)-upper limit (exclusive). Table S2. Sensitivity calculated for VAFs tested. VAFs were binned into 0.01, expressed as lower limit (inclusive)-upper limit (exclusive). Table S3. Mean minimum VAF detected for every combination of reference allele and alternate nucleotide in PIK3CA at 600 × test sample depth. Table S4. Number of variants identified by PBVI-OG, LoFreq, and Vardict variant callers. Table S5. Variants of interest identified in 27 overgrowth samples.