Background/Objectives: Human longevity is influenced both by genetic and non-genetic factors, where genetic variability accounts for 25% of human life expectancy variation (1). We aimed to elucidate SNPs that are significantly related to longevity as defined by cut-off age of 90.0 in a sample of elderly persons of European origin. Methods: 42 SNPs – selected due to strong or repeatedly found association with human longevity in other studies – were genotyped in 314 individuals aged 85.0+ from Croatia. Univariate and multivariate logistic regressions were performed with genotypic data coded as: 2 = longevity allele homozygotes ; 1 = heterozygotes ; 0 = non-longevity allele homozygotes. Results: 16 SNPs that reached inclusion criteria (p