Hydrophilic Stent Coating Inhibits Platelet Adhesion on Stent Surfaces : Initial Results In Vitro
- Resource Type
- Authors
- Hermann Monstadt; Albrecht Bufe; Marcus Peters; Pervinder Bhogal; Hans Henkes; Catrin Bannewitz; Tim Lenz-Habijan; Rosa Martinez Moreno
- Source
- Cardiovascular and Interventional Radiology
- Subject
- Anti-thrombogenic coating
Surface Properties
Scanning electron microscope
medicine.medical_treatment
Medizin
chemistry.chemical_element
In Vitro Techniques
engineering.material
Flow diverter stent
030218 nuclear medicine & medical imaging
law.invention
03 medical and health sciences
Platelet Adhesiveness
0302 clinical medicine
Coated Materials, Biocompatible
Fibrinolytic Agents
Coating
law
Materials Testing
Microscopy
Alloys
medicine
Humans
Radiology, Nuclear Medicine and imaging
Anti-platelet
business.industry
Stent
Aneurysm
Microscopy, Electron
Nickel
chemistry
Nickel titanium
Laboratory Investigation
Microscopy, Electron, Scanning
engineering
Stents
Dura Mater
Electron microscope
Cardiology and Cardiovascular Medicine
business
Hydrophobic and Hydrophilic Interactions
030217 neurology & neurosurgery
Biomedical engineering
Titanium
- Language
- English
Background: Endovascular stents and flow diverter stents (FDS) have revolutionized the treatment of intradural aneurysms; however, the need for dual anti-platelet treatment (DAPT) limits their use and can cause additional issues. Therefore, there is a need to develop stent coatings that negate the need for DAPT. Methods: Two different hydrophilic polymer coatings (HPC-I and HPC-II) were used to coat small nickel titanium plates to initially test the hydrophilic properties of these coatings when applied to nickel titanium. The plates were subsequently incubated with non-medicated whole blood from healthy volunteers for 10 min and stained with a CD61 immunofluorescent antibody that allows detection of adherent platelets. The coatings were applied to FDS wires and were again incubated with non-medicated whole blood from the same volunteers. Scanning electron microscopy was used to detect adherent platelets on the wire surface. Results: The HPC-II coating (1.12 ± 0.4%) showed a significantly lower CD61 +ve cell count (p ≤ 0.001) compared to both uncoated NiTi plates (48.61 ± 7.3%) and those with the HPC-I coating (mean 40.19 ± 8.9%). Minimal adherent platelets were seen on the FDS nickel titanium wires coated with the HPC-II compared to uncoated FDS under electron microscopy. Conclusion: There is a significant decrease in the number of adherent CD61 +ve platelets on nickel titanium surfaces coated with the HPC-II coating compared to uncoated surfaces. The coating can be successfully applied to the wires of flow diverters. The results of this study are promising with regard to the development of new anti-thrombogenic endovascular devices.